ECO-MP: E coli-Metabolic Pathway-development of Genome-scale Metabolic Pathway Database for Escherichia coli
نویسندگان
چکیده
منابع مشابه
Metabolic engineering of a 1,2-propanediol pathway in Escherichia coli.
1,2-Propanediol (1,2-PD) is a major commodity chemical that is currently derived from propylene, a nonrenewable resource. A goal of our research is to develop fermentation routes to 1,2-PD from renewable resources. Here we report the production of enantiomerically pure R-1,2-PD from glucose in Escherichia coli expressing NADH-linked glycerol dehydrogenase genes (E. coli gldA or Klebsiella pneum...
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BACKGROUND Glycolate is a valuable chemical with extensive applications in many different fields. The traditional methods to synthesize glycolate are quite expensive and toxic. So, the biotechnological production of glycolate from sustainable feedstocks is of interest for its potential economic and environmental advantages. D-Xylose is the second most abundant sugar in nature and accounts for 1...
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Recent metabolic engineering practice was briefly reviewed in particular for the useful metabolite production such as natural products and biofuel productions. With the emphasis on systems biology approach, the metabolic regulation of the main metabolic pathways in E. coli was discussed from the points of view of enzyme level (allosteric and phosphorylation/ dephosphorylation) regulation, and g...
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MetaCyc is a metabolic-pathway database (DB) that describes 445 pathways and 1115 enzymes occurring in 158 organisms. As a general reference source on metabolic pathways, MetaCyc serves several purposes in metabolic engineering. It provides an encyclopedic listing of enzymes and their properties to allow a metabolic engineer to find an enzyme whose characteristics solve an engineering problem a...
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BACKGROUND Heterologous expression systems based on promoters inducible with isopropyl-β-D-1-thiogalactopyranoside (IPTG), e.g., Escherichia coli BL21(DE3) and cognate LacI(Q)/P(lacUV5)-T7 vectors, are commonly used for production of recombinant proteins and metabolic pathways. The applicability of such cell factories is limited by the complex physiological burden imposed by overexpression of t...
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ژورنال
عنوان ژورنال: Trends in Bioinformatics
سال: 2014
ISSN: 1994-7941
DOI: 10.3923/tb.2014.7.12